SEQUENCE LISTING

<110> Amemiya, Kei
      Dimitrov, Dimiter S.
 	Xiao, Xiaodong

<120> Human Monoclonal Antibodies Protective Against Bubonic Plague

<130> 003/402/SAP

<140> 

<141> 2010-04-08

<150> US 60/212,166

<151> 2009-04-08

<160> 16

<170> Apple Macintosh Microsoft Word
		
<210> 1
<211> 17
<212> PRT
<213> Y. pestis F1 antigen peptide 
<220>
<400> 1
Met Lys Lys Ile Ser Ser Val Ile Ala Ile 
                 5                   10

Ala Leu Phe Gly Thr Ile Ala
                15

<210> 2
<211> 17
<212> PRT
<213> Y. pestis F1 antigen peptide 
<220> 
<400> 2

Val Ile Ala Ile Ala Leu Phe Gly Thr Ile 
                 5                   10

Ala Thr Ala Asn Ala Ala Asp
                15 



<210> 3
<211> 231
<212> PRT
<213> Heavy chain m252 antibody
<220> 
<400> 3

Glu Val Gln Leu Val Gln Ser Gly Ala Glu 
                  5                  10
Val Lys Lys Pro Gly Ala Ser Val Lys Val 
                 15                  20        
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                 25                  30           
Gly Tyr Tyr Met His Trp Val Arg Gln Ala 
                 35                  40           
Pro Gly Gln Gly Leu Glu Trp Met Gly Arg 
                 45                  50           
Ile Asn Pro Asn Ser Gly Gly Thr Asn Tyr 
                 55                  60           
Ala Gln Lys Phe Gln Gly Arg Val Thr Met 
                 65                  70           
Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
                 75                  80           
Met Glu Leu Ser Arg Leu Arg Ser Asp Asp 
                 85                  90           
Thr Ala Val Tyr Tyr Cys Ala Arg Glu Arg 
                 95                 100           
Tyr Gln Leu Leu Ser Asp Tyr Tyr Tyr Gly 
                105                 110           
Met Asp Val Trp Gly Gln Gly Thr Thr Val 
                115                 120           
Thr Val Ser Ser Ala Ser Thr Lys Gly Pro 
                125                 130           
Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
                135                 140           
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
                145                 150           
Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                155                 160           
Val Thr Val Ser Trp Asn Ser Gly Ala Leu 
                165                 170           
Thr Ser Gly Val His Thr Phe Pro Ala Val 
                175                 180           
Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
                185                 190

Ser Val Val Thr Val Pro Ser Ser Ser Leu 
                195                 200
Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
                205                 210
His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                215                 220
Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
                225                 230
Ser 


<210> 4
<211> 693
<212> DNA
<213> Heavy chain m252 antibody
<220> 
<400> 4

gaggtgcagc tggtacagtc tggggctgag gtgaagaagc   40	 
ctggggcctc agtgaaggtc tcctgcaagg cttctggata   80
caccttcacc ggctactata tgcactgggt gcgacaggcc  120 
cctggacaag ggcttgagtg gatgggacgg atcaacccta  160
acagtggtgg cacaaactat gcacagaagt ttcagggcag  200
ggtcaccatg accagggaca cgtccatcag cacagcctac  240
atggagctga gcaggctgag atctgacgac acggccgtgt  280
attactgtgc gagagaaagg taccagctgc tatcagacta  320
ctactacggt atggacgtct ggggccaagg gaccacggtc  360
accgtctcct cagcctccac caagggccca tcggtcttcc  400
ccctggcacc ctcctccaag agcacctctg ggggcacagc  440
ggccctgggc tgcctggtca aggactactt ccccgaaccg  480
gtgacggtgt cgtggaactc aggcgccctg accagcggcg  520
tgcacacctt cccggctgtc ctacagtcct caggactcta  560
ctccctcagc agcgtggtga ccgtgccctc cagcagcttg  600
ggcacccaga cctacatctg caacgtgaat cacaagccca  640
gcaacaccaa ggtggacaag aaagttgagc ccaaatcttg  680
tgacaaaact agt                               693


	
<210> 5
<211> 214
<212> PRT
<213> Light chain m252 antibody
<220> 
<400> 5

Asp Ile Gln Met Thr Gln Ser Pro Ser Thr 
                  5                  10
Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
                 15                  20
Ile Thr Cys Arg Ala Ser Gln Gly Ile Gly 
                 25                  30
Asn Tyr Leu Ala Trp Ile Gln Gln Lys Pro 
                 35                  40
Gly Thr Ala Pro Lys Ser Leu Val Tyr Ala
                 45                  50
Ala Ser Arg Leu Glu Ser Gly Val Pro Ser 
                 55                  60
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                 65                  70
Phe Thr Leu Thr Ile Thr Ser Leu Gln Pro 
                 75                  80
Glu Asp Phe Ala Thr Tyr Phe Cys Gln Gln 
                 85                  90
Tyr Leu Ser Tyr Pro Ile Thr Phe Gly Gln 
                 95                 100
Gly Thr Arg Leu Glu Ile Lys Arg Thr Val 
                105                 110
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro 
                115                 120
Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala 
                125                 130
Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
                135                 140
Pro Arg Glu Ala Lys Val Gln Trp Lys Val 
                145                 150
Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
                155                 160
Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
                165                 170
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr 
                175                 180
Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
                185                 190
Leu Tyr Ala Cys Glu Val Thr His Gln Gly 
                195                 200
Leu Ser Ser Pro Val Thr Lys Ser Phe Asn 
                205                 210
Arg Gly Glu Cys







<210> 6
<211> 645
<212> DNA
<213> Light chain m252 antibody
<220> 
<400> 6

gacatccaga tgacccagtc tccatccacc ctgtctgcat   40
ctgtaggaga cagagtcacc atcacttgtc gggcgagtca   80
gggcattggg aattatttag cctggattca gcagaaacca  120
gggacagccc ctaagtccct ggtctacgcc gcatctcgtt  160
tggaaagtgg ggtcccatca agattcagcg gcagtggatc  200
tgggacagat ttcactctca ccatcaccag cctgcagcct  240
gaagattttg caacctattt ctgccaacaa tatcttagtt  280
acccgatcac cttcggccaa gggacacgac tggagattaa  320
acgaactgtg gctgcaccat ctgtcttcat cttcccgcca  360
tctgatgagc agttgaaatc tggaactgcc tctgttgtgt  400
gcctgctgaa taacttctat cccagagagg ccaaagtaca  440
gtggaaggtg gataacgccc tccaatcggg taactcccag  480
gagagtgtca cagagcagga cagcaaggac agcacctaca  520
gcctcagcag caccctgacg ctgagcaaag cagactacga  560
gaaacacaaa ctctacgcct gcgaagtcac ccatcagggc  600
ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt  640
gttaa                                        645


<210> 7
<211> 17
<212> PRT
<213> Y. pestis V antigen peptide
<220> 
<400> 7
Met Ile Arg Ala Tyr Glu Gln Asn Pro Gln 
                  5                  10
His Phe Ile Glu Asp Leu Glu
                 15

<210> 8
<211> 17
<212> PRT
<213> Y. pestis V antigen peptide
<220> 
<400> 8
Glu Gln Asn Pro Gln His Phe Ile Glu Asp 
                  5                  10
Leu Glu Lys Val Arg Val Glu
                 15

<210> 9
<211> 223
<212> PRT
<213> Heavy chain m253 antibody
<220> 
<400> 9

Glu Val Gln Leu Val Gln Ser Gly Ala Glu
                  5                  10
Val Lys Lys Pro Gly Ala Ser Val Lys Val
                 15                  20
Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                 25                  30
Ser Tyr Gly Ile Ser Trp Val Arg Gln Ala 
                 35                  40
Pro Gly Gln Gly Leu Glu Trp Met Gly Trp 
                 45                  50
Ile Ser Ala Tyr Asn Gly Asn Thr Asn Tyr 
                 55                  60
Ala Gln Lys Leu Gln Gly Arg Val Thr Met 
                 65                  70
Thr Thr Asp Thr Ser Thr Ser Thr Ala Tyr 
                 75                  80
Met Glu Leu Arg Ser Leu Arg Ser Asp Asp 
                 85                  90
Thr Ala Val Tyr Tyr Cys Ala Arg Val Ala 
                 95                 100
Arg Ala Phe Asp Ile Trp Gly Gln Gly Thr 
                105                 110
Thr Val Thr Val Ser Ser Ala Ser Thr Lys 
                115                 120
Gly Pro Ser Val Phe Pro Leu Ala Pro Ser 
                125                 130
Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
                135                 140
Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                145                 150
Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
                155                 160
Ala Leu Thr Ser Gly Val His Thr Phe Pro 
                165                 170
Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
                175                 180
Leu Ser Ser Val Val Thr Val Pro Ser Ser 
                185                 190
Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
                195                 200
Val Asn His Lys Pro Ser Asn Thr Lys Val 
                205                 210
Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
                215                 220
Lys Thr Ser


<210> 10
<211> 669
<212> DNA
<213> Heavy chain m253 antibody
<220> 
<400> 10
gaagtgcagc tggtgcagtc tggagctgag gtgaagaagc   40
ctggggcctc agtgaaggtc tcctgcaagg cctctggtta   80
cacctttacc agctatggta tcagctgggt gcgacaggcc  120
cctggacaag ggcttgagtg gatgggatgg atcagcgctt  160
acaatggtaa cacaaactat gcacagaagc tccagggcag  200
agtcaccatg accacagaca catccacgag cacagcctac  240
atggagctga ggagcctgag atctgacgac acggccgtgt  280
attactgtgc gagggtggct cgggcttttg atatctgggg  320
ccaagggacc acggtcaccg tctcctcagc ctccaccaag  360
ggcccatcgg tcttccccct ggcaccctcc tccaagagca  400
cctctggggg cacagcggcc ctgggctgcc tggtcaagga  440
ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc  480
gccctgacca gcggcgtgca caccttcccg gctgtcctac  520
agtcctcagg actctactcc ctcagcagcg tggtgaccgt  560
gccctccagc agcttgggca cccagaccta catctgcaac  600
gtgaatcaca agcccagcaa caccaaggtg gacaagaaag  640
ttgagcccaa atcttgtgac aaaactagt              669         
 

<210> 11
<211> 214
<212> PRT
<213> Light chain m253 antibody
<220> 
<400> 11

Asp Thr Gln Met Thr Gln Ser Pro Ser Phe 
                 5                   10
Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
                 15                  20
Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser 
                 25                  30
Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro 
                 35                  40
Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala 
                 45                  50
Ala Ser Thr Leu Gln Ser Gly Val Pro Ser 
                 55                  60
Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu 
                 65                  70
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
                 75                  80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
                 85                  90
Leu Asn Ser Tyr Pro Leu Thr Phe Gly Pro 
                 95                 100
Gly Thr Lys Val Asp Ile Lys Arg Thr Val 
                105                 110
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro 
                115                 120
Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala 
                125                 130
Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
                135                 140
Pro Arg Glu Ala Lys Val Gln Trp Lys Val 
                145                 150
Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
                155                 160
Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
                165                 170
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr 
                175                 180
Leu Ser Lys Ala Asp Tyr Gly Lys His Lys 
                185                 190
Leu Tyr Ala Cys Glu Val Thr His Gln Gly 
                195                 200
Leu Ser Ser Pro Val Thr Lys Ser Phe Asn 
                205                 210
Arg Gly Glu Cys

<210> 12
<211> 645
<212> DNA
<213> Light chain m253 antibody
<220> 
<400> 12
gacacccaga tgacccagtc tccatccttc ctgtctgcat    40
ctgtaggaga cagagtcacc atcacttgcc gggccagtca    80
gggcattagc agttatttag cctggtatca gcaaaaacca   120
gggaaagccc ctaagctcct gatctatgct gcatccactt   160
tgcaaagtgg ggtcccatca aggttcagcg gcagtggatc   200
tgggacagaa ttcactctca caatcagcag cctgcagcct   240
gaagattttg caacttatta ctgtcaacag cttaatagtt   280
accctctcac tttcggccct gggaccaaag tggatatcaa   320
acgaactgtg gctgcaccat ctgtcttcat cttcccgcca   360
tctgatgagc agttgaaatc tggaactgcc tctgttgtgt   400
gcctgctgaa taacttctat cccagagagg ccaaagtaca   440
gtggaaggtg gataacgccc tccaatcggg taactcccag   480
gagagtgtca cagagcagga cagcaaggac agcacctaca   520
gcctcagcag caccctgacg ctgagcaaag cagactacga   560
gaaacacaaa ctctacgcct gcgaagtcac ccatcagggc   600
ctgagctcgc ccgtcacaaa gagcttcaac aggggagagt   640
gttaa                                         645


<210> 13
<211> 226
<212> PRT
<213> Heavy chain m254 antibody
<220> 
<400> 13

Glu Val Gln Leu Val Glu Ser Gly Gly Val 
                  5                  10
Val Val Gln Pro Gly Gly Ser Leu Arg Leu
                 15                  20
Ser Cys Ala Ala Ser Gly Phe Thr Phe Asp 
                 25                  30
Asp Tyr Thr Met His Trp Val Arg Gln Ala 
                 35                  40
Pro Gly Lys Gly Leu Glu Trp Val Ser Leu 
                 45                  50
Ile Ser Trp Asp Gly Gly Ser Thr Tyr Tyr 
                 55                  60
Ala Asp Ser Val Lys Gly Arg Phe Thr Ile 
                 65                  70
Ser Arg Asp Asn Ser Lys Asn Ser Leu Tyr 
                 75                  80
Leu Gln Met Asn Ser Leu Arg Thr Glu Asp 
                 85                  90
Thr Ala Leu Tyr Tyr Cys Ala Lys Gly Pro 
                 95                 100
Gly Gly Trp Tyr Tyr Phe Asp Tyr Trp Gly 
                105                 110
Gln Gly Thr Leu Val Thr Val Ser Ser Ala 
                115                 120
Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
                125                 130
Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly 
                135                 140
Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
                145                 150
Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
                155                 160
Asn Ser Gly Ala Leu Thr Ser Gly Val His 
                165                 170
Thr Phe Pro Ala Val Leu Gln Ser Ser Gly 
                175                 180
Leu Tyr Ser Leu Ser Ser Val Val Thr Val 
                185                 190
Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
                195                 200
Ile Cys Asn Val Asn His Lys Pro Ser Asn 
                205                 210
Thr Lys Val Asp Lys Lys Val Glu Pro Lys 
                215                 220
Ser Cys Asp Lys Thr Ser
                225


<210> 14
<211> 678 
<212> DNA
<213> Heavy chain m254 antibody
<220> 
<400> 14
gaggtgcagc tggtggagtc cgggggagtc gtggtacagc   40 
ctggggggtc cctgagactc tcctgtgcag cctctggatt   80 
cacctttgat gattatacca tgcactgggt ccgtcaagct  120
ccggggaagg gtctggagtg ggtctctctt attagttggg  160
atggtggtag cacatactat gcagactctg tgaagggccg  200
attcaccatc tccagagaca acagcaaaaa ctccctgtat  240
ctgcaaatga acagtctgag aactgaggac accgccttgt  280
attactgtgc aaaaggaccc ggtggctggt actactttga  320
ctactggggc cagggaaccc tggtcaccgt ctcctcagcc  360
tccaccaagg gcccatcggt cttccccctg gcaccctcct  400
ccaagagcac ctctgggggc acagcggccc tgggctgcct  440
ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg  480
aactcaggcg ccctgaccag cggcgtgcac accttcccgg  520
ctgtcctaca gtcctcagga ctctactccc tcagcagcgt  560
ggtgaccgtg ccctccagca gcttgggcac ccagacctac  600
atctgcaacg tgaatcacaa gcccagcaac accaaggtgg  640
acaagaaagt tgagcccaaa tcttgtgaca aaactagt    678


<210> 15
<211> 214
<212> PRT
<213> Light chain m254 antibody
<220> 
<400> 15

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
                  5                  10
Leu Ser Ala Ser Val Gly Asp Arg Val Thr
                 15                  20
Ile Thr Cys Arg Ala Ser Gln Gly Ile Ser 
                 25                  30
Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro
                 35                  40
Gly Lys Val Pro Lys Leu Leu Ile Tyr Ala 
                 45                  50
Ala Ser Thr Leu Gln Ser Gly Val Pro Ser
                 55                  60
Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
                 65                  70
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro
                 75                  80
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Lys 
                 85                  90
Tyr Asn Ser Ala Leu Leu Thr Phe Gly Gln
                 95                 100
Gly Thr Arg Leu Glu Ile Lys Arg Thr Val 
                105                 110
Ala Ala Pro Ser Val Phe Ile Phe Pro Pro
                115                 120
Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala 
                125                 130
Ser Val Val Cys Leu Leu Asn Asn Phe Tyr
                135                 140
Pro Arg Glu Ala Lys Val Gln Trp Lys Val 
                145                 150
Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln
                155                 160
Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
                165                 170
Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr
                175                 180
Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
                185                 190
Leu Tyr Ala Cys Glu Val Thr His Gln Gly
                195                 200
Leu Ser Ser Pro Val Thr Lys Ser Phe Asn 
                205                 210
Arg Gly Glu Cys

<210> 16
<211> 647
<212> DNA
<213> Light chain m254 antibody
<220> 
<400> 16
ccgacatcca gatgacccag tctccatcct ccctgtctgc   40 
atctgtagga gacagagtca ccatcacttg ccgggcgagt   80
cagggcatta gcaattattt agcctggtat cagcagaaac  120
cagggaaagt tcctaagctc ctgatctatg ctgcatccac  160
tttgcaatca ggggtcccat ctcggttcag tggcagtgga  200
tctgggacag atttcactct caccatcagc agcctgcagc  240
ctgaagatgt tgcaacttat tactgtcaaa agtataacag  280
tgccctcctc accttcggcc aagggacacg actggagatt  320
aaacgaactg tggctgcacc atctgtcttc atcttcccgc  360
catctgatga gcagttgaaa tctggaactg cctctgttgt  400
gtgcctgctg aataacttct atcccagaga ggccaaagta  440
cagtggaagg tggataacgc cctccaatcg ggtaactccc  480
aggagagtgt cacagagcag gacagcaagg acagcaccta  520
cagcctcagc agcaccctga cgctgagcaa agcagactac  560
gagaaacaca aactctacgc ctgcgaagtc acccatcagg  600
gcctgagctc gcccgtcaca aagagcttca acaggggaga  640
gtgttaa                                      647





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